Open AccessGenetic dissection of natural variation in oilseed traits of camelina by whole‐genome resequencing and QTL mapping
Author(s) -
Li Huang,
Hu Xiao,
Lovell John T.,
Grabowski Paul P.,
Mamidi Sujan,
Chen Cindy,
Amirebrahimi Mojgan,
Kahanda Indika,
Mumey Brendan,
Barry Kerrie,
Kudrna David,
Schmutz Jeremy,
Lachowiec Jennifer,
Lu Chaofu
Publication year - 2021
Publication title -
the plant genome
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 41
ISSN - 1940-3372
DOI - 10.1002/tpg2.20110
Subject(s) - camelina , biology , quantitative trait locus , camelina sativa , population , candidate gene , genetic variation , single nucleotide polymorphism , genetics , microbiology and biotechnology , crop , agronomy , gene , genotype , demography , sociology
Camelina [ Camelina sativa (L.) Crantz] is an oilseed crop in the Brassicaceae family that is currently being developed as a source of bioenergy and healthy fatty acids. To facilitate modern breeding efforts through marker‐assisted selection and biotechnology, we evaluated genetic variation among a worldwide collection of 222 camelina accessions. We performed whole‐genome resequencing to obtain single nucleotide polymorphism (SNP) markers and to analyze genomic diversity. We also conducted phenotypic field evaluations in two consecutive seasons for variations in key agronomic traits related to oilseed production such as seed size, oil content (OC), fatty acid composition, and flowering time. We determined the population structure of the camelina accessions using 161,301 SNPs. Further, we identified quantitative trait loci (QTL) and candidate genes controlling the above field‐evaluated traits by genome‐wide association studies (GWAS) complemented with linkage mapping using a recombinant inbred line (RIL) population. Characterization of the natural variation at the genome and phenotypic levels provides valuable resources to camelina genetic studies and crop improvement. The QTL and candidate genes should assist in breeding of advanced camelina varieties that can be integrated into the cropping systems for the production of high yield of oils of desired fatty acid composition.