Open AccessNatural variations in a pectin acetylesterase gene, MdPAE10 , contribute to prolonged apple fruit shelf life
Author(s) -
Wu Bei,
Shen Fei,
Chen Chi Jie,
Liu Li,
Wang Xuan,
Zheng Wen Yan,
Deng Yang,
Wang Ting,
Huang Zhen Yu,
Xiao Chen,
Zhou Qian,
Wang Yi,
Wu Ting,
Xu Xue Feng,
Han Zhen Hai,
Zhang Xin Zhong
Publication year - 2021
Publication title -
the plant genome
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 41
ISSN - 1940-3372
DOI - 10.1002/tpg2.20084
Subject(s) - bulked segregant analysis , biology , quantitative trait locus , shelf life , malus , flesh , single nucleotide polymorphism , candidate gene , population , gene , horticulture , genetics , food science , genotype , gene mapping , chromosome , demography , sociology
Room‐temperature shelf life is a key factor in fresh market apple ( Malus domestica Borkh.) quality and commercial value. To investigate the genetic and molecular mechanism underlying apple shelf life, quantitative trait loci (QTL) were identified using bulked segregant analysis via sequencing (BSA‐seq). Ethylene emission, flesh firmness, or crispness of apple fruit from 1,273 F 1 plants of M. asiatica Nakai ‘Zisai Pearl’ × M. domestica ‘Golden Delicious’ were phenotyped prior to and during 6 wk of room‐temperature storage. Segregation of ethylene emission and the flesh firmness or crispness traits was detected in the population. Thirteen QTL, including three major ones, were identified on chromosome 03, 08, and 16. A candidate gene encoding pectin acetylesterase, MdPAE10 , from the QTL Z16.1 negatively affected fruit shelf life. A 379‐bp deletion in the coding sequence of MdPAE10 disrupted its function. A single nucleotide polymorphism (SNP) in the MdPAE10 promoter region reduced its transcription activity. These findings provided insight into the genetic control of fruit shelf life and can be potentially used in apple marker‐assisted selection.