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Role of Notch pathway in effect of mono‐2‐ethylhexyl phthalate on the proliferation and cell cycle of SH‐SY5Y cell
Author(s) -
Zhang Bo,
Li Xu,
Zhang Xueting,
Ye Jiaming,
Zhao Weisen,
Zhang Meng,
Xing Jiqiang,
Qi Wen,
Ye Lin
Publication year - 2021
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.23314
Subject(s) - cell cycle , cell growth , apoptosis , sh sy5y , cell cycle checkpoint , microbiology and biotechnology , phthalate , notch signaling pathway , viability assay , cyclin d1 , cell , flow cytometry , chemistry , signal transduction , biology , neuroblastoma , cell culture , biochemistry , organic chemistry , genetics
Neuroblastoma (NB) is an estrogen‐dependent tumor. Mono‐2‐ethylhexyl phthalate (MEHP) has an estrogen‐like effect. However, the effects of MEHP on the progression of NB are not well illustrated. This study was to clarify the effect of Notch pathway on proliferation and cell cycle of SH‐SY5Y cell induced by MEHP. The viability of SH‐SY5Y and BE2C cells were detected by CCK8; cell cycle and apoptosis were detected by flow cytometry; the protein expression levels of Notch pathway and cell cycle related proteins were detected by Western‐blot. Results show that MEHP exposure can promote cell proliferation and altered the cell cycle. MEHP exposure can up‐regulate the expression of C‐MYC, Cyclin D1, Bcl‐2 and affected the Notch pathway. In conclusion, MEHP exposure can promote NB cell proliferation and affect the cell cycle and apoptosis. Notch pathway plays a critical role in accelerating the cell cycle and inhibiting the apoptosis of SH‐SY5Y cells caused by MEHP.