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In vitro antitumoral effects of the steroid ouabain on human thyroid papillary carcinoma cell lines
Author(s) -
Teixeira Mariana Pires,
Passos Eliza Freitas,
Haddad Natalia Ferreira,
Andrade Marcelle Novaes,
Rumjanek Vivian Mary,
MirandaAlves Leandro,
Carvalho Denise Pires,
Paiva Luciana Souza
Publication year - 2021
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.23130
Subject(s) - ouabain , medicine , cancer research , endocrinology , cancer cell , cell culture , biology , cell , vimentin , cancer , cell cycle , chemistry , microbiology and biotechnology , biochemistry , genetics , immunohistochemistry , organic chemistry , sodium
Ouabain is a steroid described as a compound extracted from plants that is capable of binding to Na + , K + ‐ATPase, inhibiting ion transport and triggering cell signaling pathways. Due to its positive ionotropic effect, ouabain was used for more than 200 years for the treatment of cardiac dysfunctions. Numerous antitumor effects of ouabain have been described so far; however, its role on thyroid cancer is still poorly understood. Therefore, the aim of the present work was to evaluate the effect of ouabain on the biology of human papillary thyroid cancer cells. For this, three human thyroid cell lines were used: NTHY‐ori, a non‐tumor lineage, BCPAP and TPC‐1, both derived from papillary carcinomas. Cells were cultured in the presence or absence of ouabain. Subsequently, we evaluated its effects on the viability, cell death, cell cycle, and migratory ability of these cell lines. We also investigated the impact of ouabain in IL‐6/IL‐6R and epithelial to mesenchymal transition markers expression. Our results indicate that ouabain (10 −7 M), decreased the number of NTHY‐ori, TPC‐1 and BCPAP viable cells and induced cell cycle arrest after in vitro culture, but did not appear to promote cell death. In TPC‐1 cells ouabain also inhibited cell migration; increased IL‐6/IL‐6R expression and IL‐6 secretion; and diminished vimentin and SNAIL‐1 expression. Collectively, our results indicate that ouabain has an antitumoral role on human papillary thyroid carcinomas in vitro. Even though additional studies are necessary, our work contributes to the discussion of the possibility of new clinical trials of ouabain.