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Enhanced anticancer potency of hydroxytyrosol and curcumin by PLGA‐PAA nano‐encapsulation on PANC ‐1 pancreatic cancer cell line
Author(s) -
Jadid Mahdieh Farhoudi Sefidan,
Shademan Behrouz,
Chavoshi Reza,
Seyyedsani Nasrin,
Aghaei Elnaz,
Taheri Elham,
Goleij Pouya,
Hajazimian Saba,
Karamad Vahidreza,
Behroozi Javad,
Sabet Mehrdad Nasrollahzadeh,
Isazadeh Alireza,
Baradaran Behzad
Publication year - 2021
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.23103
Subject(s) - curcumin , apoptosis , chemistry , pancreatic cancer , viability assay , mtt assay , pharmacology , cancer cell , cancer research , cancer , biochemistry , medicine
Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic pancreatic cancer (PC), but with only minimal improvement in survival and prognosis. Here, we investigated anti‐cancer function of free and nano‐encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd‐Cur) on PANC‐1 cell line. The poly lactide‐co‐glycolide‐co‐polyacrylic acid (PLGA‐co‐PAA) nano‐encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano‐encapsulated Hyd, Cur, and Hyd‐Cur. Effects of free and nano‐encapsulated Hyd, Cur, and Hyd‐Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC‐1 cells. We observed that free and nano‐encapsulated Hyd, Cur, and Hyd‐Cur significantly increased apoptosis rates as well as significantly decreased viability, migration, and colony formation in PANC‐1 cells. According to our results, Hyd‐Cur combination and nano‐encapsulation therapy exerts more profound apoptotic and anti‐proliferative effects on PANC‐1 cells than free Hyd or Hyd monotherapy.