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TRIB3 confers glioma cell stemness via interacting with β‐catenin
Author(s) -
Lu Yunhe,
Li Liangdong,
Chen Lei,
Gao Yang,
Chen Xin,
Cao Yiqun
Publication year - 2020
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22905
Subject(s) - glioma , gene knockdown , catenin , cancer research , biology , microbiology and biotechnology , chemistry , signal transduction , wnt signaling pathway , cell culture , genetics
Here, we aim to explore whether tribbles pseudokinase 3 (TRIB3) enhances glioma cell stemness. TRIB3 was overexpressed in glioma tissues and cell‐formed spheres, positively correlated with the size and grade. Additionally, TRIB3 expression displayed a negative correlation with the overall survival rate of glioma patients. Moreover, TRIB3 knockdown reduced the stemness of nonadherent spheres, evident by the decreased sphere‐forming ability, stemness master expression, and ALDH1 activity, while TRIB3 overexpression enhanced the stemness of adherent cells, which was rescued by β‐catenin knockdown. Mechanistically, TRIB3 activated β‐catenin signaling via physically interacting with β‐catenin. This study suggests that the TRIB3‐β‐catenin interaction is responsible for glioma cell stemness.