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Oxidative stress, immunological response, and heat shock proteins induction in the Chinese Mitten Crab, Eriocheir sinensis following avermectin exposure
Author(s) -
Hong Yuhang,
Huang Yi,
Huang Zhiqiu
Publication year - 2020
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22858
Subject(s) - eriocheir , chinese mitten crab , superoxide dismutase , glutathione , oxidative stress , catalase , glutathione peroxidase , alkaline phosphatase , acid phosphatase , hsp70 , reactive oxygen species , dismutase , lysozyme , chemistry , heat shock protein , lactate dehydrogenase , biochemistry , biology , zoology , enzyme , gene
Abstract In this study, the Chinese mitten crabs, Eriocheir sinensis were exposed to avermectin at 0.03, 0.06, 0.12, 0.24, and 0.48 mg/L respectively for 96 hours. The results showed that levels of superoxide dismutase, catalase, and glutathione peroxidase in hepatopancreas were slightly induced at concentration of 0.03 and 0.06 mg/L, but significantly ( P < .05) decreased at higher concentrations, meanwhile similar trend of the activities of acid phosphatase, alkaline phosphatase and lysozyme were observed. Significant induction of HSP70 and HSP90 mRNA expression was detected at 24 hours whereas no significant change was found in HSP60. In addition, levels of reactive oxygen species in hepatocytes increased in dose‐ and time‐ dependent manners, and cell viabilities of hepatocytes and haemocytes decreased. These results indicated that sublethal concentration exposure of avermectin had a prominent oxidative stress effect on E. sinensis based on the antioxidative and immunological activity inhibition, and HSP60, 70, and 90 perform a protective response during the exposure.