Protective effects of hepatocyte growth factor gene overexpression against hydrogen peroxide‐induced apoptosis in mesenchymal stem cells | Zendy

Choi Yong Jun | Zendy; Lee Chang Min | Zendy; Lee Jeong Hyun | Zendy; Park SeeHyoung | Zendy; Nam Myeong Jin | Zendy

Premium

Protective effects of hepatocyte growth factor gene overexpression against hydrogen peroxide‐induced apoptosis in mesenchymal stem cells

Author(s) -

Choi Yong Jun,

Lee Chang Min,

Lee Jeong Hyun,

Park SeeHyoung,

Nam Myeong Jin

Publication year - 2019

Publication title -

environmental toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.813

H-Index - 77

eISSN - 1522-7278

pISSN - 1520-4081

DOI - 10.1002/tox.22824

Subject(s) - hepatocyte growth factor , mesenchymal stem cell , apoptosis , p38 mitogen activated protein kinases , reactive oxygen species , kinase , microbiology and biotechnology , viability assay , chemistry , population , intracellular , extracellular , biology , protein kinase a , biochemistry , receptor , medicine , environmental health

Hepatocyte growth factor (HGF) has recently been reported to exhibit antioxidant and antiapoptotic effects. Therefore, we investigated the effect of overexpression of HGF gene in H 2 O 2 ‐treated mesenchymal stem cells (MSCs). HGF‐overexpression increased the cell viability from 50% to 84%, decreased the population of apoptotic cells from 20% to 16%, and decreased the intracellular reactive oxygen species (ROS) levels from 127% to 100% in cells treated with H 2 O 2 . HGF suppression decreased the cell viability from 58% to 36%, increased the population of apoptotic cells from 23 to 81%, and increased the intracellular ROS levels from 181% to 240% in cells exposed to H 2 O 2 . HGF‐overexpression also reduced the expression levels of proapoptotic proteins in MSCs treated with H 2 O 2 . Phosphorylation of extracellular signal‐regulated kinases, c‐Jun amino‐terminal kinases, and p38, which was induced by H 2 O 2 , decreased in MSCs overexpressing the HGF gene. Taken together, our results suggest that HGF has a protective effect on H 2 O 2 ‐induced apoptosis in MSCs.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research