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Protective effects of hepatocyte growth factor gene overexpression against hydrogen peroxide‐induced apoptosis in mesenchymal stem cells
Author(s) -
Choi Yong Jun,
Lee Chang Min,
Lee Jeong Hyun,
Park SeeHyoung,
Nam Myeong Jin
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22824
Subject(s) - hepatocyte growth factor , mesenchymal stem cell , apoptosis , p38 mitogen activated protein kinases , reactive oxygen species , kinase , microbiology and biotechnology , viability assay , chemistry , population , intracellular , extracellular , biology , protein kinase a , biochemistry , receptor , medicine , environmental health
Hepatocyte growth factor (HGF) has recently been reported to exhibit antioxidant and antiapoptotic effects. Therefore, we investigated the effect of overexpression of HGF gene in H 2 O 2 ‐treated mesenchymal stem cells (MSCs). HGF‐overexpression increased the cell viability from 50% to 84%, decreased the population of apoptotic cells from 20% to 16%, and decreased the intracellular reactive oxygen species (ROS) levels from 127% to 100% in cells treated with H 2 O 2 . HGF suppression decreased the cell viability from 58% to 36%, increased the population of apoptotic cells from 23 to 81%, and increased the intracellular ROS levels from 181% to 240% in cells exposed to H 2 O 2 . HGF‐overexpression also reduced the expression levels of proapoptotic proteins in MSCs treated with H 2 O 2 . Phosphorylation of extracellular signal‐regulated kinases, c‐Jun amino‐terminal kinases, and p38, which was induced by H 2 O 2 , decreased in MSCs overexpressing the HGF gene. Taken together, our results suggest that HGF has a protective effect on H 2 O 2 ‐induced apoptosis in MSCs.