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The mechanism of damage by trace amounts of acetamiprid to the midgut of the silkworm, Bombyx mori
Author(s) -
Wang Hui,
Li Fanchi,
Qu Jianwei,
Mao Tingting,
Chen Jian,
Li Mengxue,
Lu Zhengting,
Fang Yilong,
Shi Guofang,
Li Bing
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22775
Subject(s) - acetamiprid , midgut , bombyx mori , biology , chemistry , oxidative stress , toxicology , biochemistry , food science , botany , pesticide , ecology , larva , gene , imidacloprid
Acetamiprid is widely used for agricultural pest control. However, it remains poorly understood whether the environmental residues of acetamiprid have the potential effects on economic insect. In this study, we evaluated the effects of acetamiprid on silkworm growth and development. The exposure to trace amounts of acetamiprid significantly decreased body weight, viability, and spinning ability. In addition, the activity of trypsin in the midgut was decreased after exposure. DGE and KEGG pathway enrichment analysis revealed that the significantly differentially expressed genes were mainly involved in nutrient metabolism, stress responses, and inflammation pathways. These results, in combination with hematoxylin‐eosin staining and transmission electron microscopy, indicated that acetamiprid could cause oxidative damage to midgut, lead to inflammatory responses, and affect the activities of midgut digestive enzymes, thus resulting in abnormal growth and development. Our findings greatly contributed to the evaluation of the effects of acetamiprid residues on other nontarget beneficial insect.