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Diallyl disulfide attenuation effect on transcriptome in rat liver cells against cadmium chloride toxicity
Author(s) -
CobbAbdullah Ahkinyala,
Lyles Leonard Roy,
Odewumi Caroline O,
Latinwo Lekan M,
Badisa Veera Ld,
Abazinge Michael
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22766
Subject(s) - transcriptome , diallyl disulfide , cadmium chloride , toxicity , chemistry , cadmium , microbiology and biotechnology , gene expression , biochemistry , gene , biology , toxicology , apoptosis , organic chemistry
In this report, liver cells were treated with cadmium chloride (CdCl 2 ) and diallyl disulfide (DADS), a major compound from garlic to attenuate the toxic effect of Cd on transcriptome. The viability of Cd treated cells was reduced to 19.9% ± 2.4% in comparison to the untreated cells, whereas the viability of DADS pretreated cells was increased to 48.6% ± 2%. The attenuation effect of DADS was studied at shorter period (6 hours). Transcriptome analysis of CdCl 2 alone treated cells resulted in 2119 and 982 (up and down) regulated genes (≥ 2 or ≤ 2‐fold), whereas pretreated cells with DADS resulted in 2597 and 1784 genes. These genes were known to function in many important biological processes. Affymetrix array analysis was validated by the pathway specific PCR array that exhibited the same trend of expression. The current study clearly shows the DADS attenuation effect on transcriptome in CdCl 2 ‐treated rat liver cells.