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The effect of naringenin on the role of nuclear factor (erythroid‐derived 2)‐like2 (Nrf2) and haem oxygenase 1 (HO‐1) in reducing the risk of oxidative stress‐related radiotoxicity in the spleen of rats
Author(s) -
AbdelMagied Nadia,
Shedid Shereen M.
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22745
Subject(s) - chemistry , malondialdehyde , superoxide dismutase , oxidative stress , glutathione , glutathione peroxidase , catalase , heme oxygenase , ionizing radiation , pharmacology , medicine , biochemistry , irradiation , enzyme , heme , physics , nuclear physics
The present study was to evaluate the radiomitigative effect of naringenin (NRG) on the modulation of ionizing radiation (IR)‐induced spleen injury. Rats were exposed to 12 Gy (3Gy/two times/week). NRG (50mg/Kg), was orally given one hour after the first radiation dose, and daily continued during the irradiation period. Rats were sacrificed 1 day after the last dose of radiation. NRG showed a significant decrease of malondialdehyde, hydrogen peroxide with a significant elevation of superoxide dismutase, catalase and glutathione peroxidase activities and glutathione content. Moreover, NRG confirmed the intracellular defense mechanisms through activation of nuclear factor (erythroid‐derived 2)‐like2 ( Nrf2 ) and haem oxygenase‐1 (HO‐1) levels and their protein expression. In addition, NRG deactivated the nuclear factor‐κB (NF‐κB) and reduced the pro‐inflammatory cytokines. Further, NRG showed positive modulation in the haematological values (WBCs, RBCs, Hb, Hct% and PLt). In conclusion, these results suggested that NRG reversed the IR‐induced redox‐imbalance in the rat spleen.