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Chronic exposure to dichloroacetamide induces biochemical and histopathological changes in the gills of zebrafish
Author(s) -
Zhang Yu,
Sun HongJie,
Zhang JingYing,
Ndayambaje Emmanuel,
Lin Hongjun,
Chen Jianrong,
Hong Huachang
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22744
Subject(s) - gill , superoxide dismutase , malondialdehyde , atpase , zebrafish , lipid peroxidation , enzyme , antioxidant , biology , chemistry , biochemistry , toxicology , fishery , fish <actinopterygii> , gene
Abstract To evaluate the impact of DCAcAm on zebrafish gill, we measure the responses of antioxidant enzyme (superoxide dismutase, SOD), lipid peroxidation (malondialdehyde, MDA), ATPase (Na + /K + ‐ATPase and Ca 2+ /Mg 2+ ‐ATP) and histopathological changes of gill in adult zebrafish, after exposed to different concentrations of DCAcAm (0, 1, 10, 100, and 1000 μg L −1 ) for 30 days. Results indicated that DCAcAm first increased and then decreased SOD activity, and DCAcAm also lowered the activities of Na + /K + ‐ATPase and Ca 2+ /Mg 2+ ‐ATPase. These results indicated that high affinity of DCAcAm probably be a main factor, which can damage the structures of enzymes, thereby inhibiting the SOD and ATPase activities. Besides, histopathological investigation results also manifested that chronic exposure to DCAcAm can damage the gill tissues, disrupting the normal function of gills. We conclude that chronic exposure to DCAcAm was harmful to organisms, not only influence gill function, but also further cause damage on the gill tissues.