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Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF‐κB signaling pathway in vitro
Author(s) -
Chen HsinYu,
Jiang YiWen,
Kuo ChaoLin,
Way TzongDer,
Chou YuCheng,
Chang YuanShiun,
Chung JingGung
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22697
Subject(s) - chrysin , protein kinase b , chemistry , cancer research , cell migration , pi3k/akt/mtor pathway , apoptosis , mtt assay , cell culture , cancer cell , cell growth , cell , pharmacology , biology , medicine , cancer , biochemistry , genetics , flavonoid , antioxidant
Abstract Numerous evidences have shown that chrysin induced cytotoxic effects via induced cell cycle arrest and induction of cell apoptosis in human cancer cell lines, however, no information showed that chrysin inhibited skin cancer cell migration and invasion. In this study, we investigated anti‐metastasis mechanisms of chrysin in human melanoma cancer A375.S2 cells in vitro. Under sub‐lethal concentrations of chrysin (0, 5, 10, and 15 μM) which inhibits cell mobility, migration and invasion of A375.S2 cells that were assayed by wound healing and Transwell filter. That chrysin inhibited MMP‐2 activity in A375.S2 cells was investigated by gelatin zymography assay. Western blotting was used to examine protein expression and results indicated that chrysin inhibited the expression of GRB2, SOS‐1, PKC, p‐AKT (Thr308), NF‐κBp65, and NF‐κBp50 at 24 and 48 hours treatment, but only at 10‐15 μM of chrysin decreased Ras, PI3K, p‐c‐Jun, and Snail only at 48 hours treatment and only decrease p‐AKT(Ser473) at 24 hours treatment. Furthermore, chrysin (5‐15 μM) decreased the expression of uPA, N‐cadherin and MMP‐1 at 24 and 48 hours treatment but only decreased MMP‐2 and VEGF at 48 hours treatment at 10‐15 μM and 5‐15 μM of chrysin, respectively, however, increased E‐cadherin at 5‐15 μM treatment. Results of confocal laser microscopy systems indicated that chrysin inhibited expression of NF‐κBp65 in A375.S2 cells. Based on these observations, we suggest that chrysin can be used in anti‐metastasis of human melanoma cells in the future.