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Kaempferol suppresses cell migration through the activation of the ERK signaling pathways in ARPE‐19 cells
Author(s) -
Chien HsiangWen,
Wang Kai,
Chang YuanYen,
Hsieh YiHsien,
Yu NuoYi,
Yang ShunFa,
Lin HuiWen
Publication year - 2019
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22686
Subject(s) - kaempferol , mapk/erk pathway , signal transduction , microbiology and biotechnology , kinase , cell migration , phosphorylation , chemistry , extracellular matrix , retinal pigment epithelium , biology , cell , flavonoid , biochemistry , retinal , antioxidant
Abstract Kaempferol is a flavonoid with anticancer and anti‐metastasis activity in different cancer‐cell lines. However, the underlying mechanisms by which kaempferol acts on human retinal pigment epithelial (ARPE‐19) cells remain unclear. In this study, we demonstrated that kaempferol inhibited migration and invasion in ARPE‐19 cells at non‐toxic dosages. We discovered that kaempferol obviously reduced the enzyme activity and protein expression of matrix metalloproteinase‐2 by increasing the phosphorylated levels of extracellular signal‐regulated kinases 1/2 (ERK1/2) signaling pathways. Additionally, ERK1/2‐specific inhibitor PD98059 significantly reversed kaempferol's inhibitory effects on migration and expression of MMP‐2 in ARPE‐19 cells. Overall, our results are the first to demonstrate that kaempferol is capable of inhibiting cell migration by targeting ERK1/2 signaling in human retinal pigment epithelial cells.