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Effects of novel brominated flame retardant TBBPA on human airway epithelial cell (A549) in vitro and proteome profiling
Author(s) -
Wu Shijin,
Wu Mei,
Qi Mengting,
Zhong Li,
Qiu Lequan
Publication year - 2018
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22632
Subject(s) - tetrabromobisphenol a , viability assay , lipid peroxidation , reactive oxygen species , brominated flame retardant , chemistry , a549 cell , cell , in vitro , toxicity , pharmacology , oxidative stress , microbiology and biotechnology , fire retardant , biochemistry , biology , organic chemistry
The cellular toxicity response of human airway epithelial cells (A549) to tetrabromobisphenol (TBBPA) was assessed in vitro. Cell viability, levels of intracellular reactive oxygen species (ROS), lipid peroxidation (MDA), and caspase‐3 activity were determined after A549 treated with varying concentrations of TBBPA. A comparative proteomic analysis was performed in cells treated with different concentrations of TBBPA (0, 10, and 40 μg/mL). Two‐way anova analysis showed that cell viability was significantly decreased after treatment by TBBPA with a concentration of 16 μg/mL for 48 hr, however, the caspase‐3 activities, ROS generation, and MDA content increased. Ultrastructural observation revealed that the cell was morphological damaged after exposure to 64 μg/mL TBBPA, with mitochondria seriously injured and the smooth endoplasmic reticulum dilated. There was a good correlation between ROS generation and mitochondrial dysfunction. Seventeen differentially expressed proteins involved in various biological processes were identified. These findings provide a basis for understanding the mechanisms of cell dysfunction and perturbation of antioxidant status induced by additive flame retardant on airway epithelial cells.