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Polyphenols from brown alga, Padina boergesenii (Allendar & Kraft) decelerates renal cancer growth involving cell cycle arrest and induction of apoptosis in renal carcinoma cells
Author(s) -
Rajamani Karthikeyan,
Thirugnanasambandan Somasundaram S.
Publication year - 2018
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22619
Subject(s) - apoptosis , polyphenol , cell cycle checkpoint , cancer cell , cell cycle , chemistry , cytotoxicity , growth inhibition , cell culture , cancer , cell growth , biochemistry , biology , cancer research , antioxidant , medicine , in vitro , genetics
In our previous work, we assessed the ameliorative effect of crude extract from Padina boergesenii . In this report, we have extended these study by fractionating the methanol extract with methanol:ethylacetate (1:3,v/v) (MME‐F) and assessed the cytotoxic effect of MME‐F fraction in human renal carcinoma cell lines (A498 and ACHN). The fraction had time‐and dose‐dependent inhibition of cancer cell proliferation, migration with deceleration of cancer growth at EC 50 —22.73 μg in A498 and 26.43 μg in ACHN cells. Cells treated at EC 50 value 25 μg displayed twofold greater ability to induce early and late stage of apoptosis. The cells treated with polyphenolic fraction (MME‐F) induced cell cycle arrest at G2/M phase. HPLC/DAD chromatographic procedures quantified polyphenols from active fraction (MME‐F). These data revealed the functional activity of polyphenols from brown alga, P. boergesenii as a potent inhibitor of cancer proliferation with induction of apoptosis, it suggest their applicability in preventing cancer metastasis.