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Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats
Author(s) -
Chiang JuiTing,
Badrealam Khan Farheen,
Shibu Marthandam Asokan,
Kuo ChiaHua,
Huang ChihYang,
Chen BihCheng,
Lin YuehMin,
Viswanadha Vijaya Padma,
Kuo WeiWen,
Huang ChihYang
Publication year - 2018
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22589
Subject(s) - eriobotrya , muscle hypertrophy , in vivo , medicine , blot , western blot , endocrinology , angiotensin ii , h&e stain , japonica , biology , blood pressure , biochemistry , botany , immunohistochemistry , microbiology and biotechnology , gene
Eriobotrya japonica (EJ) is a traditional Chinese plant with high medicinal value. EJ extracts are reported to exhibit antioxidant and anti‐inflammatory biological attributes. The current study aims to evaluate the prospective efficacy of E. japonica leave extract (EJLE) against Angiotensin‐II induced cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats (SHRs). For the in vitro studies, Angiotensin‐II pretreated H9c2 cells were treated with EJLE and analyzed through Western blotting and rhodamine phalloidin staining for their cardio‐protective attributes. In the in vivo studies, 12‐week‐old SHRs were randomly divided into groups: SHRs supplemented with EJLE, control SHR group supplemented with PBS; in addition, a control group of Wistar–Kyoto rats (WKY) was also employed. All rats were supplemented twice a week for 8 week time interval. Finally, echocardiography, morphological, histology, and Western blot analysis were performed to assess their role against cardiac hypertrophy. Interestingly, we could observe that supplementation of EJLE could rescue Ang‐II induced cardiac hypertrophy as evident through Western blot, rhodamine phalloidin staining, and Hematoxylin‐Eosin staining. Notably, morphological and echocardiography data provided further supports for their ability to ameliorate cardiac characteristics. Cumulatively, the results clearly suggests that supplementation of EJLE promotes cardio‐protective effects through amelioration of cardiac hypertrophy in vitro and in vivo.