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Osthole induces human nasopharyngeal cancer cells apoptosis through Fas–Fas ligand and mitochondrial pathway
Author(s) -
Liu PeiYing,
Chang DunCheng,
Lo YuSheng,
Hsi YiTing,
Lin ChiaChieh,
Chuang YiChing,
Lin ShuHui,
Hsieh MingJu,
Chen MuKuan
Publication year - 2018
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22530
Subject(s) - apoptosis , fadd , nasopharyngeal carcinoma , fas ligand , blot , cell culture , mtt assay , flow cytometry , poly adp ribose polymerase , caspase 8 , cancer research , caspase , signal transduction , microbiology and biotechnology , fas receptor , biology , cell growth , chemistry , programmed cell death , biochemistry , medicine , polymerase , enzyme , genetics , gene , radiation therapy
Nasopharyngeal carcinoma (NPC) is endemic in Southern China and Southeast Asia. The present study investigated the activity of osthole in suppressing NPC along with the underlying mechanism. Cell growth inhibition was measured using the MTT assay. Apoptosis was detected through 4ʹ,6‐diamidino‐2‐phenylindole staining and flow cytometry. Western blotting was used to identify the signaling pathway. Osthole markedly inhibited cell proliferation and induced apoptosis in the NPC cell line. Western blotting results revealed the increased activation of caspases 3, 8, and 9 and poly (ADP‐ribose) polymerase. Osthole treatment significantly reduced the expression of the antiapoptotic protein Bcl‐2 and increased the expression of the proapoptotic proteins Bax, Bak, BimL, BimS, and t‐Bid. Osthole treatment also increased the expression of Fas, FADD, TNF‐R1, TNF‐R2, DcR2, RIP, and DR5. In addition, osthole treatment significantly increased the expression levels of phosphorylated ERK1/2 and JNK1/2. These results suggested that osthole exerts cytotoxic effects on NPC cell lines mainly through apoptosis mediated by the Fas–Fas ligand and mitochondrial pathway. Osthole could be a potential anticancer agent for NPC.