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Crocin and quercetin prevent PAT ‐induced apoptosis in mammalian cells: Involvement of ROS ‐mediated ER stress pathway
Author(s) -
Boussabbeh Manel,
Prola Alexandre,
Ben Salem Intidhar,
Guilbert Arnaud,
Bacha Hassen,
Lemaire Christophe,
AbisEssefi Salwa
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22185
Subject(s) - reactive oxygen species , oxidative stress , lipid peroxidation , chemistry , malondialdehyde , antioxidant , biochemistry , dna damage , crocin , pharmacology , apoptosis , toxicity , unfolded protein response , patulin , biology , food science , mycotoxin , dna , organic chemistry
ABSTRACT Patulin (PAT) is a secondary metabolite produced by several species of the genera of Penicillium , Aspergillus , and Byssochlamys that can be found in rotting fruits, especially in apples and apple‐based products. Exposure to this mycotoxin has been reported to induce intestinal and kidney injuries. The mechanism underlying such toxicity has been linked to the induction of apoptosis which occurred with reactive oxygen species production and endoplasmic reticulum (ER) stress induction. This study aimed to evaluate the effect of the two common dietary compounds Quercetin (QUER), a natural flavonoid, and Crocin (CRO), a natural carotenoid, on PAT‐induced toxicity in human colon carcinoma (HCT116) and embryonic kidney cells (HEK293). We showed that antioxidant properties of QUER and CRO help to prevent ER stress activation and lipid peroxidation as evidenced by the reduction in GRP78 and GADD34 expressions and the decrease in malondialdehyde production. Furthermore, we demonstrated their ability to re‐establish the loss of the mitochondrial membrane potential to inhibit caspase 3 activation and DNA fragmentation. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1851–1858, 2016.