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The N rf2‐ ARE pathway is associated with S chisandrin b attenuating benzo(a)pyrene‐Induced HTR cells damages in vitro
Author(s) -
Dong Qulong,
Hou Haiyan,
Wu Jun,
Chen Yaqiong
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22149
Subject(s) - benzo(a)pyrene , cytotoxicity , chemistry , in vitro , western blot , cytoprotection , microbiology and biotechnology , pyrene , apoptosis , biochemistry , biology , organic chemistry , gene
As is ubiquitous in the environmental sources, benzo(a)pyrene (BaP) has been reported to induce reprotoxicity in previous studies. Toxicity to trophoblast cells may be one key factor, but evidences were absent. We speculated that BaP can induce cytotoxicity in human trophoblast HTR‐8/SVneo (HTR) cells, and Schisandrin B (Sch B) as a potential protector can inhibit the cytotoxicity. MTS assay identified that BaP induced HTR cells death while Sch B played a cytoprotective role. And after Nrf2 interference, the ability of Sch B‐induced cytoprotection was declined. Furthermore, PCR, western blot, ELISA, and SOD assays were found that Sch B significantly increased the mRNA and protein expression of Nrf2, HO1, NQO1, and SOD in the Nrf2‐ARE pathway, and the extents of increase were declined after Nrf2 interference. These results demonstrated that the Nrf2‐ARE pathway plays an important role in Sch B attenuating BaP‐induced HTR cells damages in vitro . © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1439–1449, 2016.