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Oxidative damage of naphthenic acids on the E isenia fetida earthworm
Author(s) -
Wang Jie,
Cao Xiaofeng,
Chai Liwei,
Liao Jingqiu,
Huang Yi,
Tang Xiaoyan
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22139
Subject(s) - eisenia fetida , catalase , lipid peroxidation , genotoxicity , superoxide dismutase , comet assay , oxidative stress , glutathione peroxidase , earthworm , chemistry , dna damage , antioxidant , ecotoxicity , glutathione , toxicology , environmental chemistry , biochemistry , toxicity , enzyme , biology , dna , ecology , organic chemistry
Naphthenic acids (NAs) have been gaining recognition in recent years as potentially harmful environmental contaminants. Few studies have focused on the potential ecotoxicity of NAs to terrestrial environment. In this study, the responses of antioxidant system and lipid peroxidation and DNA damage were investigated after exposing Eisenia fetida to soil contaminated with NAs. The results indicated that NAs induced a significant increase ( p < 0.05) in superoxide dismutase and catalase enzyme activities. The glutathione peroxidase enzyme activities were significantly inhibited ( p < 0.05) in the medium and high dose treatments. An increase in malondialidehyde indicated that NAs could cause cellular lipid peroxidation in the tested earthworms. The percentage of DNA in the tail of comet assay of coelomocytes as an indication of DNA damage increased after treatment with different doses of NAs, and a dose‐dependent DNA damage of coelomocytes was found. In conclusion, oxidative stress caused by NAs exposure induces physiological responses and genotoxicity on earthworms. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1337–1343, 2016.