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Inhibition of H e L a cells metastasis by bioactive compounds in crocodile ( C rocodylus siamensis ) white blood cells extract
Author(s) -
Patathana Supawadee,
Thammasirirak Sompong,
Daduang Jureerut,
Gung Chung Jing,
Temsiripong Yosapong,
Daduang Sakda
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22138
Subject(s) - matrix metalloproteinase , hela , angiogenesis , apoptosis , metastasis , chemistry , microbiology and biotechnology , cell , biology , cancer research , biochemistry , cancer , genetics
Matrix metalloproteinases (MMPs) play a key role in cancer progression, including cell invasion, metastasis, cell growth, apoptosis, angiogenesis, and cell adhesion. Thus, suppression of the MMPs activities is crucial for inhibiting cancer cells metastasis. Herein, bioactive agents from crocodile ( Crocodylus siamensis ) leukocyte extracts (WBCex) showed the anticancer activity with HeLa cells and inhibited the migration and invasion process by reducing gelatinases (MMP‐2, MMP‐9) activity and their protein levels. This mechanism is regulated via interfering Ras and p38 signal transduction. Moreover, disrupting VEGF and integrin‐signaling cascade by bioactive agents are the predictable mechanisms that cause the decreasing of MMP‐2 and MMP‐9 activity. Hence, bioactive substances in WBCex may play the mode of action similar with MMPs inhibitor due to HeLa cell metastasis being suppressed in vitro . © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1329–1336, 2016.