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The possible role of liver kinase B 1 in hydroquinone‐induced toxicity of murine fetal liver and bone marrow hematopoietic stem cells
Author(s) -
Li Zhen,
Wang Chunhong,
Zhu Jie,
Bai YuE,
Wang Wei,
Zhou Yanfeng,
Zhang Shaozun,
Liu Xiangxiang,
Zhou Sheng,
Huang Wenting,
Bi Yongyi,
Wang Hong
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22094
Subject(s) - haematopoiesis , stem cell , bone marrow , biology , hematopoietic stem cell , apoptosis , microbiology and biotechnology , cancer research , immunology , biochemistry
Epidemiological studies suggest that the increasing incidence of childhood leukemia may be due to maternal exposure to benzene, which is a known human carcinogen; however, the mechanisms involved remain unknown. Liver Kinase B1 (LKB1) acts as a regulator of cellular energy metabolism and functions to regulate hematopoietic stem cell (HSC) homeostasis. We hypothesize that LKB1 contributes to the deregulation of fetal or bone hematopoiesis caused by the benzene metabolite hydroquinone (HQ). To evaluate this hypothesis, we compared the effects of HQ on murine fetal liver hematopoietic stem cells (FL‐HSCs) and bone marrow hematopoietic stem cells (BM‐HSCs). FL‐HSCs and BM‐HSCs were isolated and enriched by a magnetic cell sorting system and exposed to various concentrations of HQ (0, 1.25, 2.5, 5, 10, 20, and 40 μM) for 24 h. We found that the inhibition of differentiation and growth, as well as the apoptosis rate of FL‐HSCs, induced by HQ were consistent with the changes in BM‐HSCs. Furthermore, G1 cell cycle arrest was observed in BM‐HSCs and FL‐HSCs in response to HQ. Importantly, FL‐HSCs were more sensitive than BM‐HSCs after exposure to HQ. The highest induction of LKB1 and adenosine monophosphate‐activated protein kinase (AMPK) was observed with a much lower concentration of HQ in FL‐HSCs than in BM‐HSCs. LKB1 may play a critical role in apoptosis and cell cycle arrest of HQ‐treated HSCs. This research has developed innovative ideas concerning benzene‐induced hematopoietic toxicity or embryotoxicity, which can provide a new experimental evidence for preventing childhood leukemia. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 830–841, 2016.