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Anti‐apoptotic effect of S an H uang S hel S hin T ang cyclodextrin complex (SHSSTc) on CCl 4 ‐induced hepatotoxicity in rats
Author(s) -
Yang ChengHsun,
Ting WeiJen,
Shen ChiaYao,
Hsu HsiHsien,
Lin YuehMin,
Kuo ChiaHua,
Tsai FuuJen,
Tsai ChangHai,
Tsai Yuhsin,
Huang ChihYang
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22078
Subject(s) - baicalein , pharmacology , apoptosis , chemistry , tunel assay , fadd , liver injury , scutellaria baicalensis , coptis chinensis , intraperitoneal injection , protein kinase b , traditional medicine , programmed cell death , caspase , medicine , traditional chinese medicine , biochemistry , alternative medicine , pathology
ABSTRACT The metabolic loading is heavier in liver especially when injured or inflammation. San Huang Shel Shin Tang (SHSST) was an old traditional herbal decoction, which composed with Rheum officinale Baill , Scutellaria baicalnsis Geprgi and Coptis chinensis Franch (1:1:2 in weight), can provide a liver protection effects. We used a beta‐cyclodextrin (β‐CD) drug modification method in reduce of the necessary dose of the SHSST. As the results, the FAS‐FADD expressions leaded apoptosis in CCl 4 intraperitoneal (IP) injection induced acute liver injury in rats. Silymarin, baicalein, SHSST, and SHSST β‐CD complex (SHSSTc) pretreatments protected liver through the decreasing of the expressions of FAS‐FADD and downstream caspase‐3 and caspase‐8. Particularly, SHSSTc (30 mg/kg day) treatment enhanced cell survival pathway activation through the PI3K, Akt and Bad phosphorylation. Compared with SHSST as well as silymarin and baicalein, SHSSTc provided a magnificent liver protection effect, especially in survival pathway activation/TUNEL‐apoptotic cell reduction/serum cholesterol level suppression. All these data suggested that β‐CD complex modified the SHSST and promoted the bioavailability and liver protection effects. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 663–670, 2016.