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Docosahexaenoic acid and l ‐Carnitine prevent ATP loss in SH‐SY5Y neuroblastoma cells after exposure to silver nanoparticles
Author(s) -
Tan Joey WeeShan,
Ho Christabel FungYih,
Ng YeeKong,
Ong WeiYi
Publication year - 2016
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22037
Subject(s) - docosahexaenoic acid , sh sy5y , mitochondrion , carnitine , chemistry , incubation , biochemistry , membrane potential , silver nanoparticle , inner mitochondrial membrane , microbiology and biotechnology , biology , cell culture , fatty acid , nanoparticle , neuroblastoma , nanotechnology , polyunsaturated fatty acid , materials science , genetics
Silver nanoparticles (AgNPs) are among the most commonly used nanomaterials, but thus far, little is known about ways to mitigate against potential toxic effects of exposure. In this study, we examined the potential effects of AgNPs on mitochondrial function and cellular ATP levels, and whether these could be prevented by treatment with docosahexaenoic acid (DHA) and l ‐carnitine (LC). Acute exposure of AgNPs for 1 h to SH‐SY5Y cells resulted in decreased mitochondrial membrane potential, and decreased ATP and ADP levels, indicating mitochondrial damage and reduced production of ATP. Incubation of cells with DHA partially reduced, while treatment with LC and DHA completely abolished the AgNP induced decreases in ATP and ADP levels. This could be due to a LC‐facilitated entry of DHA to mitochondria, for repair of damaged phospholipids. It is postulated that DHA and LC may be useful for treatment of accidental environmental exposure to AgNPs. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 224–232, 2016.