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Cytotoxic, genotoxic, and neurotoxic effects of Mg, Pb, and Fe on pheochromocytoma (PC‐12) cells
Author(s) -
Sanders Talia,
Liu YiMing,
Tchounwou Paul B.
Publication year - 2015
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.22014
Subject(s) - genotoxicity , oxidative stress , comet assay , neurotoxicity , toxicity , viability assay , chemistry , dna damage , cytotoxicity , dopamine , pharmacology , cytotoxic t cell , carcinogen , toxicology , microbiology and biotechnology , biochemistry , biology , cell , in vitro , endocrinology , dna , organic chemistry
Metals such as lead (Pb), magnesium (Mg), and iron (Fe) are ubiquitous in the environment as a result of natural occurrence and anthropogenic activities. Although Mg, Fe, and others are considered essential elements, high level of exposure has been associated with severe adverse health effects including cardiovascular, hematological, nephrotoxic, hepatotoxic, and neurologic abnormalities in humans. In the present study we hypothesized that Mg, Pb, and Fe are cytotoxic, genotoxic and neurotoxic, and their toxicity is mediated through oxidative stress and alteration in protein expression. To test the hypothesis, we used the pheochromocytoma (PC‐12) cell line as a neuro cell model and performed the LDH assay for cell viability, Comet assay for DNA damage, Western blot for oxidative stress, and HPLC‐MS to assess the concentration levels of neurological biomarkers such as glutamate, dopamine (DA), and 3‐methoxytyramine (3‐MT). The results of this study clearly show that Mg, Pb, and Fe, respectively in the form of MgSO 4 , Pb(NO 3 ) 2 , FeCl 2 , and FeCl 3 induce cytotoxicity, oxidative stress, and genotoxicity in PC‐12 cells. In addition, exposure to these metallic compounds caused significant changes in the concentration levels of glutamate, dopamine, and 3‐MT in PC‐12 cells. Taken together the findings suggest that MgSO 4 , Pb(NO 3 ) 2 , FeCl 2 , and FeCl 3 have the potential to induce substantial toxicity to PC‐12 cells. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1445–1458, 2015.