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Chemoprotective effect of monoisoamyl 2, 3‐dimercaptosuccinate (MiADMS) on cytokines expression in cadmium chloride treated human lung cells
Author(s) -
Odewumi Caroline O.,
FilsAime Shiela,
Badisa Veera L. D.,
Latinwo Lekan M.,
Ruden Michael L.,
Ikediobi Christopher,
Badisa Ramesh B.
Publication year - 2014
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.21948
Subject(s) - viability assay , cadmium chloride , chemoprotective , cytokine , chemistry , proinflammatory cytokine , cadmium , microbiology and biotechnology , pharmacology , andrology , cell , immunology , biochemistry , inflammation , biology , medicine , enzyme , organic chemistry
Cadmium is commercially profitable element, but it causes toxicity in humans and animals leading to diseases in various organs. The main route of cadmium exposure to humans is through inhalation. Lungs respond to insult through secretion of cytokines. In this study, the chemoprotective effect of monoisoamyl 2, 3‐dimercaptosuccinate (MiADMS) was evaluated on viability and cytokines expression in CdCl 2 treated human lung A549 cells by cytokine array. Cells were treated with 0, 50, 75, and 100 µM CdCl 2 alone, 300 µM MiADMS alone, and co‐treated with 300 µM MiADMS and 75 µM CdCl 2 for 24 h. The viability was measured by crystal violet dye. The level of cytokines in the cells' lysate and cell culture medium was measured using Ray Biotech's Human Cytokine Array 6 in control cells, 75 µM CdCl 2 alone and MiADMS co‐treated cells. Array results were validated by ELISA kit. The CdCl 2 caused a dose dependent decrease in cell viability, while MiADMS co‐treatment resulted in a significant increase in viability of CdCl 2 treated cells. Morphology of the cells treated with CdCl 2 was destroyed, while MiADMS restored the lost shape in CdCl 2 treated cells. In addition, the cells co‐treated with MiADMS and CdCl 2 showed modulation of cytokines expression in comparison to the CdCl 2 alone treated cells. The ELISA results showed the similar pattern of cytokine expression as Human Cytokine Array and validated the array results. These results clearly show the chemoprotective effect of MiADMS and suggest that MiADMS can be used as antidote at moderate dose against CdCl 2 toxicity. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 704–711, 2015.