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The crude extract of Corni Fructus inhibits the migration and invasion of U‐2 OS human osteosarcoma cells through the inhibition of matrix metalloproteinase‐2/‐9 by MAPK signaling
Author(s) -
Liao ChingLung,
Lin JuHwa,
Lien JinCherng,
Hsu ShuChun,
Chueh FuShin,
Yu ChienChih,
Wu PingPing,
Huang YiPing,
Lin JaungGeng,
Chung JingGung
Publication year - 2015
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.21894
Subject(s) - osteosarcoma , matrix metalloproteinase , cancer research , pi3k/akt/mtor pathway , chemistry , mapk/erk pathway , population , cancer , medicine , apoptosis , signal transduction , biochemistry , environmental health
Osteosarcoma is the most common primary malignancy of the bone cancers. In the Chinese population, the crude extract of Corni Fructus (CECF) has been used as Traditional Chinese medicine to treat several different diseases for hundreds of years. In the present study, effects of CECF on inhibition of migration and invasion in U‐2 OS human osteosarcoma cells were examined. CECF significantly inhibited migration and invasion of U‐2 OS human osteosarcoma cells. We also found that CECF inhibited activities of matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9). CECF decreased protein levels of FAK, PKC, SOS1, MKK7, MEKK3, GRB2, NF‐κB p65, COX‐2, HIF‐1α, PI3K, Rho A, ROCK‐1, IRE‐1α, p‐JNK1/2, p‐ERK1/2, p‐p38, Ras, p‐PERK, MMP‐2, MMP‐9, and VEGF in U‐2 OS cells. Results of this study indicate that CECF may have potential as a novel anticancer agent for the treatment of osteosarcoma by inhibiting migration and invasion of cancer cells © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 53–63, 2015.