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Heavy metal influence on BDE‐47 uptake in the human KERTr keratinocyte cell line
Author(s) -
Kang Yuan,
Yin Yi,
Zhang Qiu Yun,
Li Lai Sheng,
Zeng Li Xuan,
Luo Ji Wen,
Wong Ming Hung
Publication year - 2014
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.21849
Subject(s) - keratinocyte , cell culture , metal , chemistry , heavy metals , environmental chemistry , microbiology and biotechnology , biophysics , biology , organic chemistry , genetics
Significant correlations between concentrations of PBDEs and heavy metals were observed in the human body. However, there is a lack of evidence on the linkage between the uptake of heavy metals and PBDEs. This study is the first report on the BDE‐47 uptake profile in a human cell line. Hg and As exposures to KERTr (human skin derived keratinocyte) did not significantly ( p > 0.05) affect the uptake of BDE47, whereas Pb and Cd significantly ( p < 0.05) affected the uptake of BDE‐47 in KERTr. The change in K m was minor after exposure to all heavy metals. The maximum transport rate ( V max ) after exposure to Pb ( V max : 5.23 ± 0.49) and As ( V max : 4.95 ± 0.60) was significantly increased when compared with the background of the KERTr cell line ( V max : 4.07 ± 0.35). Real‐time RT‐PCR indicated that OATP‐B, OATP‐D, and OATP‐E were expressed in the KERTr cell line. The upregulation or downregulation of OATP B and D genes were minor after exposure to heavy metals, but the OATP E gene was upregulated by three to fourfold in KERTr cell line after exposure to Pb an Cd, which may explain the significant increase of uptake of BDE‐47 in KERTr after exposures to Pb and Cd. This study indicated that the uptake effects should be considered when performing risk assessment of human exposure to PBDEs and heavy metal. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 354–361, 2014.