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Cytochrome P450 system expression and DNA adduct formation in the liver of Zacco platypus following waterborne Benzo( a )pyrene exposure: implications for biomarker determination
Author(s) -
Lee Jin Wuk,
Kim Yong Hwa,
Yoon Seokjoo,
Lee Sung Kyu
Publication year - 2014
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.21833
Subject(s) - benzo(a)pyrene , biomarker , cytochrome p450 , pyrene , toxicity , biology , dna damage , carcinogen , chemistry , dna , microbiology and biotechnology , biochemistry , toxicology , enzyme , organic chemistry , astrobiology
Benzo( a )pyrene (B a P) is a polycyclic aromatic hydrocarbon that causes mutations and tumor formation. Zacco platypus is a sentinel species that is suitable for monitoring aquatic environments. We studied cytochrome P450 system (CYP system) expression and DNA adduct formation in the liver of Z. platypus following waterborne exposure to B a P. The results showed both dose and time dependency. The significant induction levels of CYP system mRNA and protein reached maximums at 2 days and 14 days, respectively, and hepatosomatic index was maximally induced at 4 days during 14 days B a P exposure. DNA adduct formation was significantly induced compared to corresponding controls ( t ‐test, p < 0.01) after 4 days of exposure in 100 μg/L B a P. These results indicate that the only use of mRNA expression level of CYP system as a biomarker make us underestimate prolonged toxicity (4–14 days) of B a P and the only use of protein expression level of CYP system make us underestimate acute toxicity (1–2 days) of B a P. Therefore, we suggests that a combinational use of the mRNA expression level and protein expression level of CYP system, hepatosomatic index is a useful biomarker in risk assessment of waterborne B a P exposure. In addition, DNA adduct formation was a useful biomarker in risk assessment of waterborne B a P exposure at 4 days. CYP1A was a more sensitive biomarker than CYP reductase for B a P exposure when considering both the mRNA and protein level. Furthermore, our results show that Z. platypus is a useful species for assessing the risk of waterborne B a P exposure. 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 1032–1042, 2014.