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Chlorpyrifos induced testicular damage in rats: Ameliorative effect of glutathione antioxidant
Author(s) -
Elsharkawy Eman E.,
Yahia Doha,
ElNisr Neveen A.
Publication year - 2014
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.21831
Subject(s) - glutathione , lipid peroxidation , antioxidant , glutathione reductase , chemistry , oxidative stress , endocrinology , corn oil , superoxide dismutase , alkaline phosphatase , medicine , chlorpyrifos , biochemistry , biology , enzyme , glutathione peroxidase , pesticide , agronomy
This study investigated the induction of oxidative stress in the testes of adult rats exposed to chlorpyrifos (CPF). CPF was administered orally, in a dose of 30 mg/kg body weight to male rats for 90 days, twice weekly. Coadministration of water‐soluble nonenzymatic antioxidant glutathione (GSH) was performed in a dose of 100 mg/kg body weight, orally, for the same period. Another two groups of male rats were administered GSH and corn oil, respectively. The activities of superoxide dismutase and GSH reductase were decreased while the levels of lipid peroxidation were increased in the testicular tissues of the exposed animals. Testosterone level in the serum was significantly decreased. A decrease in the histochemical determination of testicular alkaline phosphatase was observed in CPF‐treated rats. A significant decrease in all stages of spermatogenesis in the seminiferous tubules was recorded in the exposed animals. Coadministration of GSH restored these parameters. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 1011–1019, 2014.