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Impairment of the mitochondrial oxidative phosphorylation system and oxidative stress in liver of crucian carp ( Carassius auratus L.) exposed to microcystins
Author(s) -
Zhao Yanyan,
Xie Ping,
Fan Huihui,
Zhao Sujuan
Publication year - 2014
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20770
Subject(s) - crucian carp , oxidative stress , oxidative phosphorylation , reactive oxygen species , biology , antioxidant , mitochondrion , biochemistry , chemistry , microbiology and biotechnology , fishery , fish <actinopterygii>
Hepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC‐LReq μg/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial‐encoded genes ( cox1, cox2, cox3, and atp6 ) were significantly reduced in a time‐dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. © 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30–39, 2014.