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Metals (Ag + , Cd 2+ , Cr 6+ ) affect ATPase activity in the gill, kidney, and muscle of freshwater fish Oreochromis niloticus following acute and chronic exposures
Author(s) -
Atli Gülüzar,
Canli Mustdafa
Publication year - 2013
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20766
Subject(s) - oreochromis , atpase , metal , toxicity , gill , environmental chemistry , metal toxicity , chemistry , freshwater fish , kidney , metal ions in aqueous solution , biology , nuclear chemistry , enzyme , heavy metals , fish <actinopterygii> , toxicology , biochemistry , endocrinology , fishery , organic chemistry
Freshwater fish Oreochromis niloticus were individually acutely exposed to different concentrations (0, 0.1, 0.5, 1.0, and 1.5 μg/mL) of Cd 2+ , Cr 6+ , and Ag + for 96 h and 0.05 μg/mL concentration of the same metals for different periods (0, 5, 10, 20, and 30 days) chronically. Following each experimental protocol, Na + /K + ‐ATPase, Mg 2+ ‐ATPase, and Ca 2+ ‐ATPase activities were measured in the gill, kidney, and muscle of O. niloticus . In vitro experiments were also performed to determine the direct effects of metal ions (0, 0.1, 0.5, 1.0, and 1.5 μg/mL) on ATPases. Except Ag + , none of the metals caused fish mortality within 30 days. Silver killed all the fishes within 16 days. Metal exposures generally decreased Na + /K + ‐ATPase and Ca 2+ ‐ATPase activities in the tissues of O. niloticus , although there were some fluctuations in Mg 2+ ‐ATPase activity. Ag + and Cd 2+ were found to be more toxic to ATPase activities than Cr 6+ . It was also observed that metal efficiency was higher in the gill than in the other tissues. Results indicated that the response of ATPases varied depending on metals, exposure types, and tissues. Because ATPases are sensitive to metal toxicity, their activity can give valuable data about fish physiology. Therefore, they may be used as a sensitive biomarker in environmental monitoring in contaminated waters. © 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 707–717, 2013.