Premium
Bio‐distribution and in vivo antioxidant effects of cerium oxide nanoparticles in mice
Author(s) -
Hirst Suzanne Marie,
Karakoti Ajay,
Singh Sanjay,
Self William,
Tyler Ron,
Seal Sudipta,
Reilly Christopher M.
Publication year - 2013
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20704
Subject(s) - oxidative stress , in vivo , chemistry , antioxidant , pharmacology , toxicity , cerium oxide , carbon tetrachloride , oral administration , biochemistry , biology , microbiology and biotechnology , organic chemistry , catalysis
Cerium oxide nanoparticles have oxygen defects in their lattice structure that enables them to act as a regenerative free radical scavenger in a physiological environment. We performed a comprehensive in vivo analysis of the biological distribution and antioxidant capabilities of nanoceria administered to mice perorally (PO), intravenously (IV), or intraperitoneally (IP) by dosing animals weekly for 2 or 5 weeks with 0.5 mg kg −1 nanoceria. Next, we examined if nanoceria administration would decrease ROS production in mice treated with carbon tetrachloride (CCl 4 ). Our results showed that the most extensive and cumulative nano‐deposition was via IV and IP administered while PO administration showed mice excreted greater than 95% of their nanoceria within 24 h. Organ deposition for IV and IP mice was greatest in the spleen followed by the liver, lungs, and kidneys. Elimination for all administration routes was through feces. Nanoceria administration showed no overt toxicity, however, WBC counts were elevated with IV and IP administration. Our in vivo studies show that nanoceria administration to mice with induced liver toxicity (by CCl 4 ) showed similar findings to mice treated with N ‐acetyl cystine (NAC), a common therapeutic to reduce oxidative stress. Taken together, our studies show that nanoceria remains deposited in tissues and may decrease ROS, thereby suggesting that cerium oxide nanoparticles may be a useful antioxidant treatment for oxidative stress. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2013.