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The potential of synthetic thiourea compound to reduce the cytotoxic and genotoxic effects of paraquat in Hordeum vulgare and cultured human lymphocytes
Author(s) -
Gateva Svetla,
Jovtchev Gabriele,
Stergios Mila,
Yonova Petranka
Publication year - 2012
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20633
Subject(s) - genotoxicity , micronucleus test , paraquat , hordeum vulgare , clastogen , chromosome aberration , micronucleus , biology , cytotoxicity , chemistry , pharmacology , biochemistry , in vitro , botany , toxicity , chromosome , gene , poaceae , organic chemistry
This work evaluated the ability of one synthetic compound 1‐(4‐fluorophenylthiocarbamoyl)‐4‐methyl‐piperazine (FTMP), thiourea derivative to reduce cytoxic and genotoxic effects of free radical inducer paraquat (PQ) in two different test systems Hordeum vulgare and human lymphocytes in vitro . The mitotic index was used as a marker for cytotoxicity. To indicate genotoxicity, chromosome aberrations test and micronucleus induction test were used. FTMP manifested a weak genotoxic effect in both test systems. Clear evidence was obtained that conditioning treatment with FTMP (10 −6 , 5 × 10 −6 , and 10 −5 mol/l) could decrease chromosome aberrations and micronuclei induced by PQ in both test systems. “Aberration hot spots” in heterochromatin containing segments were reduced. The present data show that the thiourea synthetic compound FTMP provides genome protection against the harmful action of oxidative stress inductor PQ. Human lymphocytes were found to be more sensitive to the cytotoxic and clastogenic effects of FTMP conditioning treatment than Hordeum vulgare . Revealing the protective action of newly synthesized compounds could contribute to the improvement of our present knowledge of the mechanisms of mutagenesis and antimutagenesis. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.