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Arsenic exposure induces genomic hypermethylation
Author(s) -
Majumdar Sunipa,
Chanda Sarmishtha,
Ganguli Bhaswati,
Mazumder D. N. Guha,
Lahiri Sarbari,
Dasgupta Uma B.
Publication year - 2010
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20497
Subject(s) - arsenic , dna methylation , methylation , genomic dna , methionine , peripheral blood mononuclear cell , methyltransferase , incubation , dna , biology , microbiology and biotechnology , peripheral blood , gene , chemistry , genetics , biochemistry , gene expression , in vitro , immunology , organic chemistry , amino acid
Gene‐specific hypermethylation has previously been detected in Arsenic exposed persons. To monitor the level of whole genome methylation in persons exposed to different levels of Arsenic via drinking water, DNA was extracted from peripheral blood mononuclear cells of 64 persons. Uptake of methyl group from 3 H labeled S‐Adenosyl Methionine after incubation of DNA with SssI methylase was measured. Results showed statistically significant ( P = 0.0004) decrease in uptake of 3 H methyl group in the persons exposed to 250–500 μg/L arsenic, indicating genomic hypermethylation. © 2009 Wiley Periodicals, Inc. Environ Toxicol, 2010.