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Comparative effect of melatonin and vitamin E on phenylhydrazine‐induced toxicity in the spleen of Funambulus pennanti
Author(s) -
Sharma Shweta,
Haldar Chandana
Publication year - 2009
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20383
Subject(s) - melatonin , chemistry , phenylhydrazine , toxicity , lipid peroxidation , medicine , endocrinology , oxidative stress , spleen , antioxidant , vitamin e , vitamin , biochemistry , pharmacology , biology , organic chemistry , medicinal chemistry
Phenylhydrazine (PHZ) oxidation resulting in free iron release followed by free radical generation has increased frequency of cancer. This study aims towards the dose‐dependent response of PHZ and the role of melatonin in comparison with vitamin E following PHZ‐induced toxicity within the lymphoid tissue (spleen) of Indian tropical seasonal breeder, Funambulus pennanti , during reproductively active phase. An increase in the damages in terms of lipid peroxidation (LPO), apoptosis percentage, and splenomegaly was observed following different doses of PHZ treatment, i.e., 0.025, 0.5, and 1 mg/100 g body weight (b.wt.), where dose of 1 mg/100 g b.wt. showed more significant damages. Both melatonin (0.5 mg/100 g b.wt.) and vitamin E (1 mg/100 g b.wt.) administration ameliorated oxidative damages of 1 mg/100 g b.wt. PHZ‐treated group. Melatonin altered PHZ‐induced responses significantly to a greater degree than vitamin E as evidenced by LPO status, SOD activity, and ABTS radical cation scavenging activity of antioxidants. Thus, melatonin might be able to restrict carcinogenic property of PHZ‐induced oxidative stress by protecting macromolecules of the cell from harmful effects of PHZ and instead preserving cell viability. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.