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Application of a new bioassay technique using goldfish for assessment of water toxicity
Author(s) -
Deguchi Yuya,
Wu Nan Xiang,
Toyoizumi Tomoyasu,
Masuda Shuichi,
Nagaoka Hiroaki,
Watanabe Tetsushi,
Totsuka Yukari,
Wakabayashi Keiji,
Kinae Naohide
Publication year - 2008
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20379
Subject(s) - genotoxicity , micronucleus test , vitellogenin , bioassay , environmental chemistry , fish <actinopterygii> , toxicity , aquatic environment , ecotoxicology , oryzias , chemistry , contamination , environmental science , chemical toxicity , toxicology , dna damage , biology , water pollutants , fishery , dna , ecology , biochemistry , organic chemistry
There are a variety of chemicals in aquatic environment, so it is important to assess the toxicity. The biomarkers such as induction of DNA damage, micronuclei, vitellogenin, and hepatic P450 in fish are known to be effective for monitoring genotoxic and/or estrogenic chemicals. However, there is little study to use these biomarkers in same fish. Goldfish ( Carassius auratus ) is widely used and is suitable in size to collect blood or organs. In this study, validity of multiple‐biomarkers in goldfish was checked using standard chemicals and applied in the river water. Ho River, which flows through the textile dyeing factory in Shizuoka Prefecture, Japan, was reported to show genotoxicity toward Salmonella typhimurium TA98 and YG1024. When the goldfish were exposed to Ho River, DNA damage, estrogenic activity, and CYP1A induction were observed. Through the study, it was assumed that not only mutagens/carcinogens but also endocrine disrupting chemicals and poly aromatic hydrocarbons were present in Ho River. Therefore, chemical identification should be required. We could evaluate both genotoxicity and estrogenic activity simultaneously, so goldfish might be a good experimental model for estimation of chemical contamination levels in aquatic environment. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.