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Transcriptional responses of xenobiotic metabolizing enzymes, HSP70 and Na + /K + ‐ATPase in the liver of rabbitfish ( Siganus oramin ) intracoelomically injected with amnesic shellfish poisoning toxin
Author(s) -
Wang Lin,
Liang XuFang,
Huang Yan,
Li ShiYing,
Ip KokChao
Publication year - 2008
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20350
Subject(s) - biology , microbiology and biotechnology , glutathione s transferase , hsp70 , toxin , glutathione , biochemistry , enzyme , heat shock protein , gene
Amnesic shellfish poisoning toxin domoic acid (DA) is a marine neurotoxin that accumulates in fish and shellfish, and has been implicated to be involved in human and marine wildlife mortality. The transcriptional responses of cytochrome P‐450 1A (CYP1A), glutathione S‐transferase alpha (GSTA), glutathione S‐transferase rho (GSTR), heat shock protein 70 (HSP70), and Na + /K + ‐ATPase alpha 1 (ATP1A1) in the liver of rabbitfish ( Siganus oramin ) intracoelomically injected with DA, were investigated. Experimental fish were administered with one injection of DA (2 μg/g wet weight) or PBS as control. After 24 h, fish were killed and hepatic RNA was isolated. Partial cDNA of rabbitfish CYP1A, GSTA, GSTR, HSP70, ATP1A1, and β‐actin were obtained by PCR using degenerate primers. Using β‐actin as an external control, the relative liver CYP1A, GSTA, GSTR, HSP70, and ATP1A1 mRNA abundance of rabbitfish were determined by semi‐quantitative RT‐PCR within the exponential phase. The ratio CYP1A/β‐actin mRNA (%) of exposure group was determined to be 148.92 ± 12.69, whereas the ratio of control group was 82.3 ± 8.35, indicating that CYP1A was induced significantly in rabbitfish following DA exposure ( P < 0.05). Although the expressions of GSTA, HSP70, and ATP1A1 tended to increase and GSTR tended to decrease, no significant changes were found ( P > 0.05). The induction of hepatic CYP1A in response to DA suggests a potential role for fish phase I xenobiotic metabolizing enzyme in DA metabolism. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.