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Application of DNA diffusion assay in earthworm coelomocytes
Author(s) -
Apte A. A.,
Manerikar R. S.,
Ghole V. S.
Publication year - 2008
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20336
Subject(s) - earthworm , apoptosis , cypermethrin , necrosis , microbiology and biotechnology , dna , chromium , tunel assay , dna damage , chemistry , diffusion , biology , toxicology , biochemistry , pesticide , genetics , ecology , organic chemistry , physics , thermodynamics
We have applied the DNA diffusion assay proposed by Singh (2000) Exp Cell Res 256:328–337, for quantitative estimation of apoptosis in earthworm coelomocytes, exposed to Chromium (VI) and cypermethrin as model toxicants in vitro . The DNA diffusion assay was originally described for mammalian cells. H 2 O 2 , Sodium ascorbate, and hyperthermia were used as positive controls in present study. Apoptosis such as DNA diffusion occurred in dose‐dependent manner for Chromium (VI) and cypermethrin at very low concentration (1, 3, and 10 ppm for Chromium (VI) and 4, 8, and 16 ppm for cypermethrin). Three distinct patterns (apoptosis like DNA diffusion, necrosis, and normal) were observed in exposed and nonexposed cells. Present study is probably the first report of application of the DNA diffusion technique in earthworm coelomocytes. Findings of this study indicate that this assay has potential for use in invertebrate cells to differentiate between apoptosis and necrosis. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.