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Effects of tobacco compounds on gene expression in fetal lung fibroblasts
Author(s) -
Sohn SungHwa,
Kim KiNam,
Kim In kyoung,
Lee EunIl,
Ryu JaeJun,
Kim MeyoungKon
Publication year - 2008
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20335
Subject(s) - nicotine , gene expression , microbiology and biotechnology , microarray analysis techniques , complementary dna , biology , microarray , cell culture , real time polymerase chain reaction , gene , apoptosis , chemistry , biochemistry , genetics , neuroscience
The goal of this study was to determine the effects of tobacco compounds on gene expression in a human fetal lung cell line (WI38). In the present study, we investigated the effects of tobacco compounds (nicotine, benzo( a )pyrene (B( a )P), and 2‐Naphthylamine) on gene expression profiles in human fetal fibroblasts using cDNA microarray and real‐time PCR. WI38 cells were cultured in Eagle's minimum essential medium (MEM) supplemented with 10% fetal bovine serum, 2% 200 mM L ‐glutamine, and a 2% penicillin and streptomycin solution. Tissue culture flasks (T‐25 cm 2 ) containing confluent lung fibroblasts were incubated at 37°C for 24 h with 5 mL of medium supplemented with 10 μM of a tobacco compound (nicotine, B( a )P, or 2‐Naphthylamine). The gene expression profiles for the W138 cells varied depending on the tobacco compound. The cDNA microarray analysis revealed that apoptosis‐related genes such as DNASE2 , MADD , MST1 , NME3 , RARG , TNFRSF1A , BAD , and DFFB genes were down‐regulated in tobacco compound‐treated WI38 cells. We also observed significant increases in Arnt gene expression by real‐time PCR in tobacco compound‐treated WI38 cells. Tobacco compounds can affect apoptosis, immunity, and growth in WI38 cells. A microarray‐based genomic survey is a high‐throughput approach for the evaluation of gene expression in cell lines treated with tobacco compounds. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.