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Adduction of DNA with MTBE and TBA in mice studied by accelerator mass spectrometry
Author(s) -
Yuan Y.,
Wang H. F.,
Sun H. F.,
Du H. F.,
Xu L. H.,
Liu Y. F.,
Ding X. F.,
Fu D. P.,
Liu K. X.
Publication year - 2007
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20295
Subject(s) - chemistry , adduct , alcohol , methyl tert butyl ether , ether , mass spectrometry , dna , methanol , dna adduct , medicinal chemistry , organic chemistry , chromatography , biochemistry
Abstract Methyl tert ‐butyl ether (MTBE) is a currently worldwide used octane enhancer substituting for lead alkyls and gasoline oxygenate. Our previous study using doubly 14 C‐labeled MTBE [(CH 3 ) 3 14 CO 14 CH 3 ] has shown that MTBE binds DNA to form DNA adducts at low dose levels in mice. To elucidate the mechanism of the binding reaction, in this study, the DNA adducts with singly 14 C‐labeled MTBE, which was synthesized from 14 C‐methanol and tert ‐butyl alcohol (TBA), or 14 C‐labeled TBA in mice have been measured by ultra sensitive accelerator mass spectrometry. The results show that the methyl group of MTBE and tert ‐butyl alcohol definitely form adducts with DNA in mouse liver, lung, and kidney. The methyl group of MTBE is the predominant binding part in liver, while the methyl group and the tert ‐butyl group give comparable contributions to the adduct formation in lung and kidney. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 630–635, 2007.