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Analysis of cyanobacterial‐derived saxitoxins using high‐performance ion exchange chromatography with chemical oxidation/fluorescence detection
Author(s) -
Papageorgiou John,
Nicholson Brenton C.,
Linke Thomas A.,
Kapralos Con
Publication year - 2005
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20144
Subject(s) - cyanobacteria , environmental chemistry , high performance liquid chromatography , shellfish poisoning , chromatography , chemistry , matrix (chemical analysis) , ion chromatography , fluorescence , anabaena , ion exchange , detection limit , shellfish , ion , biology , bacteria , aquatic animal , organic chemistry , fish <actinopterygii> , quantum mechanics , fishery , genetics , physics
A single run HPLC method utilizing ion exchange as the separation mode with a novel mobile phase system coupled to chemical postcolumn oxidation and fluorescence detection has been developed and demonstrated to be applicable to the quantitative analysis of paralytic shellfish poisons (PSPs) produced by Australian cyanobacteria ( Anabaena circinalis ) and other cyanobacteria. Both the cyanobacterial matrix and natural water constituents did not significantly affect the performance of this method. The daily precision of this method was adequate for it to be considered as a routine analytical tool for direct PSP analysis (prePSP concentration is not required) of cyanobacterial extracts and water bodies containing PSPs (C1, C2, GTX2, GTX3, NEO, STX) in the low parts per billion concentration range (10–70 ppb). © 2005 Wiley Periodicals, Inc. Environ Toxicol 20: 549–559, 2005.