Premium
Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography–mass spectrometry
Author(s) -
Chan Melissa P. L.,
Mohd Mustafa Ali
Publication year - 2005
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.20076
Subject(s) - mass spectrometry , endosulfan , chromatography , gas chromatography , gas chromatography–mass spectrometry , chemistry , environmental chemistry , pesticide , biology , ecology
A method has been developed for the determination of trace levels of α‐endosulfan, β‐endosulfan, endosulfan sulfate, and endosulfan diol in rat plasma and tissue samples. Endosulfan and its metabolites in the plasma samples were extracted with solid‐phase extraction Chromabond‐end‐capped C18 cartridges and analyzed by a Shimadzu QP‐5050A gas chromatograph–mass spectrometer (GCMS) with quadrupole detector in selected‐ion‐monitoring mode. The analysis of endosulfan and its metabolites in liver and kidney samples involved solvent extraction, Florisil solid‐phase‐extraction cleanup, and quantitation by GCMS. Recovery experiments for the plasma and tissue samples were conducted over concentration ranges of 10–100 ng mL −1 and 100–1000 ng mL −1 , respectively. The method was applied to the analysis of trace levels of endosulfan and its metabolites in plasma and tissue samples collected from an animal study. Trace levels of α‐endosulfan and β‐endosulfan in the ranges of undetectable to 3.11 μg g −1 and undetectable to 1.19 μg g −1 , respectively, were detected in the kidney samples, whereas trace levels of endosulfan sulfate in the range of 0.02–0.22 μg g −1 were detected in the liver samples of rats. Neither endosulfan nor its metabolites was detected in any of the plasma samples. © 2005 Wiley Periodicals, Inc. Environ Toxicol 20: 45–52, 2005.