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Tests for the toxicity assessment of cyanobacterial bloom samples
Author(s) -
Tarczynska Małgorzata,
NaleczJawecki Grzegorz,
RomanowskaDuda Zdzislawa,
Sawicki Jozef,
Beattie Keneth,
Codd Geoff,
Zalewski Maciej
Publication year - 2001
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.1047
Subject(s) - daphnia magna , microcystin , cyanobacteria , bioassay , microcystis , algal bloom , biology , bloom , daphnia , ecotoxicology , eutrophication , environmental chemistry , toxicity , ecology , zooplankton , chemistry , phytoplankton , bacteria , nutrient , organic chemistry , genetics
Cyanobacterial (blue–green algal) blooms are one of the common consequences of the increasing eutrophication of surface waters. The production of cyanobacterial toxins and their presence in drinking and recreational waters represents a growing danger to human and animal health. Due to a lack of toxin standards and to resource limitations on the wide‐scale use of analytical methods (e.g., high‐performance liquid chromatography, enzyme‐linked immunosorbent assay (ELISA)) in cyanobacterial toxin monitoring, it is necessary to assess and to develop additional methods for their detection and estimation. Microbiotests using invertebrates offer a possible approach for the inexpensive and straightforward detection and assessment of cyanobacterial bloom toxicity. Three microbiotests with: Thamnocephalus platyurus, Daphnia magna , and Spirostomum ambiguum were examined with bloom samples containing hepatotoxic microcystin‐LR and up to five additional microcystin variants. Two kinds of cyanobacterial bloom sample preparations were tested: crude extracts (CE) and purified extracts (PE). The highest toxicity was found when CE was used for microbiotests. The sensitivity of microorganisms decreased from S. ambiguum to T. platyurus and to D. magna . A statistically significant correlation was found between microcystin concentration and T. platyurus biotest, and between mouse bioassay and S. ambiguum results. Addition of Me 2 SO (1%, v/v) is a possible method to increase the sensitivity of the microorganisms for microcystin‐LR. © 2001 John Wiley & Sons, Inc. Environ Toxicol 16: 383–390, 2001