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Coupling toxicokinetics and pharmacodynamics for predicting survival of abalone ( Haliotis diversicolor supertexta ) exposed to waterborne zinc
Author(s) -
Liao ChungMin,
Chen BoChing,
Lin MingChao,
Chiu HsiaoMei,
Chou YunHua
Publication year - 2002
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.10082
Subject(s) - abalone , toxicokinetics , toxicity , zinc , acute toxicity , pharmacodynamics , bioconcentration , biology , toxicology , ecotoxicology , environmental chemistry , zoology , chemistry , bioaccumulation , ecology , pharmacokinetics , fishery , pharmacology , organic chemistry
Abstract We developed a mortality model, by coupling an acute toxicity model and a pharmacodynamic model, to predict survival of abalone ( Haliotis diversicolor supertexta ) exposed to waterborne zinc (Zn). We conducted a laboratory 14‐day exposure experiment to obtain biokinetic parameters of depuration rate constant ( k 2 ) and bioconcentration factor (BCF). A one‐compartment uptake–depuration model was used to fit the exposure data to estimate BCF and k 2 values. The acute toxicity model was developed based on the receptor theory and was verified with LC 50 ( t ) data obtained from a 7‐day acute toxicity test. A highly significant correlation ( r 2 = 0.98) was found between predictions and LC 50 ( t ) data for the acute toxicity model, indicating a successful description of 7‐day LC 50 ( t ) data of Zn in abalone. The predicted time course of lethal body burden of Zn in abalone was compared with measured data, showing that the average percent error was 14.04 ± 3.02%. A refined pharmacodynamic model was expressed as the Hill equation, which in terms of waterborne Zn and LC 50 ( t ) data was used to fit observed mortality percentages to determine the Hill coefficient ( r 2 = 0.98). The proposed mortality model in terms of whole body burden and lethal body burden at site of action was then employed to predict the time‐varying mortality of abalone exposed to various Zn concentrations in pond water. Our results demonstrate that 96‐h LC 50 and incipient LC 50 for H. diversicolor supertexta exposed to Zn are 1.1 and 1.05 mg L −1 , respectively. Our predictions also demonstrate that equilibrium lethal body burden at site of action is about 198 μg g −1 , whereas the mortalities never reach 50% when H. diversicolor supertexta exposed to Zn is ⩽ 1 mg L −1 . © 2002 Wiley Periodicals, Inc. Environ Toxicol 17: 478–486, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/tox.10082