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Determination of chiral ratio of o,p ‐DDT and o,p ‐DDD pesticides on polysaccharides chiral stationary phases by HPLC under reversed‐phase mode
Author(s) -
Ali Imran,
AboulEnein Hassan Y.
Publication year - 2002
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/tox.10069
Subject(s) - acetonitrile , enantiomer , chemistry , chromatography , high performance liquid chromatography , pesticide , resolution (logic) , chiral column chromatography , analytical chemistry (journal) , stereochemistry , biology , artificial intelligence , computer science , agronomy
Abstract A simple and reliable HPLC method for the chiral resolution of o,p ‐DDT and o,p ‐DDD is described. The enantiomeric resolution of o,p ‐DDT and o,p ‐DDD has been achieved on Chiralpak AD‐R, Chiralcel OD‐R, and Chiralcel OJ‐R chiral stationary phases. The mobile phases used were acetonitrile–water (50:50 [v/v]) and acetonitrile‐2‐propanol (50:50 [v/v]) at a flow rate of 1.0 mL/min. For both pesticides detection was done at 220 nm. The values for o,p ‐DDT of α and R s varied from 1.24 to 2.52 and from 0.80 to 2.47, respectively. The values of α and R s for o,p ‐DDD were 1.26 and 0.60, respectively. © 2002 Wiley Periodicals, Inc. Environ Toxicol 17: 329–333, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/tox.10069