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The active ingredients in the anti‐obesity drug bofutsushosan in high‐fat‐diet‐fed obese mice
Author(s) -
Takahashi Tatsuo,
Hori Kenta,
Yoshida Eri,
IkedaMatsuo Yuri,
Shimada Tsutomu,
Nomura Masaaki,
Kobayashi Shinjiro
Publication year - 2020
Publication title -
traditional and kampo medicine
Language(s) - English
Resource type - Journals
ISSN - 2053-4515
DOI - 10.1002/tkm2.1253
Subject(s) - gardenia , traditional medicine , glycyrrhiza , chemistry , rhizome , gum acacia , medicine , pharmacology , food science , traditional chinese medicine , alternative medicine , pathology
Aim Combined effects on the body weights and white adipose tissues in high‐fat‐diet‐fed obese mice due to bofutsushosan (BTS) were investigated. Methods Male ICR mice (four weeks old) were fed a 60% kcal fat diet for five weeks and fed the same diet with administration of aqueous extracts (2.0 g/kg/day) of BTS, component‐removed BTS, and a mixture of the ingredients and the component‐removed BTS for 28 days. Results The BTS extract reduced the body weight from 2 to 28 days after administration while also reducing the weight of the subcutaneous and visceral white adipose tissues after administration for 28 days. Extracts of BTS with Glycyrrhizae radix ( Glycyrrhiza root; abbreviated as glycyrrhiza) removed, Rhei rhizoma (rhubarb rhizome; rhubarb) removed, Gardeniae fructus ( Gardenia fruit; gardenia) removed, Ephedrae herba ( Ephedra herb; ephedra) removed, or Sal mirabilis (sodium sulfate hydrate; mirabilite) removed reduced the anti‐obesity and fat‐loss activity compared to that of the BTS extract after administration for 28 days. Glycyrrhizic acid (0.89% [w/w]) and 0.046%(w/w) sennoside AB calcium salt ameliorated the anti‐obesity activity of BTS with glycyrrhiza removed and rhubarb removed eight days after administration, respectively. Geniposide (0.78% [w/w]) and 0.16% (w/w) ephedrine hydrochloride ameliorated the anti‐obesity and fat‐loss activity of BTS with gardenia removed and with ephedra removed respectively after administration for 28 days. Conclusion Glycyrrhiza, rhubarb, gardenia, ephedra and mirabilite were the active components responsible for the effect of BTS. Glycyrrhizic acid and sennoside AB induced the early phase while geniposide and ephedrine hydrochloride induced the later phase of the anti‐obesity activity of BTS.