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Eppikajutsuto (a Japanese herbal medicine) regulates mTOR and induces apoptosis in oral cancer cells
Author(s) -
Makita Haruna,
Endo Kazuhira,
Shirai Akiko,
Kawasaki Kanji,
Mishima Rei,
Kasahara Yoshiya,
Ishikawa Kazuya,
Ueno Takayoshi,
Nakanishi Yosuke,
Kondo Satoru,
Wakisaka Naohiro,
Yoshizaki Tomokazu,
OgawaOchiai Keiko
Publication year - 2020
Publication title -
traditional and kampo medicine
Language(s) - English
Resource type - Journals
ISSN - 2053-4515
DOI - 10.1002/tkm2.1244
Subject(s) - kampo , pi3k/akt/mtor pathway , apoptosis , in vivo , in vitro , viability assay , cancer , medicine , cell growth , cancer research , cell culture , pharmacology , traditional medicine , chemistry , biology , biochemistry , pathology , genetics , alternative medicine , microbiology and biotechnology
Aim The effectiveness of Japanese traditional (Kampo) medicine is gradually attracting attention worldwide. It is typically known to be effective against cancer as a supportive therapy, but the antitumor effect of Kampo formulas is not fully established. We therefore evaluated the antitumor effect of eppikajutsuto. Methods We evaluated the antitumor effect of eppikajutsuto by cell viability assay, western blotting, and apoptotic cell assay using an oral squamous cell carcinoma cell line OSC‐19. Results We found that the administration of eppikajutsuto to OSC‐19 in vitro inhibited cell proliferation, induced apoptosis, and suppressed mTOR activation. Conclusions The results suggested that eppikajutsuto might exhibit antitumor effect against OSC‐19 in vitro by suppressing mTOR activation. However, additional studies in vitro and in vivo are required to confirm the antitumor effect of eppikajutsuto.