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Rokumigan alleviates oxaliplatin‐induced cold hypersensitivity by suppression of transient receptor potential cation channel subfamily M member 8 and subfamily A member 1
Author(s) -
Shimizu Kanako,
Kato Yoshinori,
Nakamura Hiroyoshi,
Yamada Harumi
Publication year - 2019
Publication title -
traditional and kampo medicine
Language(s) - English
Resource type - Journals
ISSN - 2053-4515
DOI - 10.1002/tkm2.1205
Subject(s) - trpm8 , transient receptor potential channel , oxaliplatin , cold sensitivity , ankyrin , chemistry , subfamily , stimulation , pharmacology , receptor , microbiology and biotechnology , medicine , biology , trpv1 , biochemistry , colorectal cancer , gene , mutant , cancer
Aim Oxaliplatin (Oxp) is a chemotherapeutic agent that is used to treat advanced or recurrent colorectal cancer. Oxp causes cold hypersensitivity in the acute therapy stage, however, by overexpression of the transient receptor potential cation channel subfamily melastatin, member 8 (TRPM8) and subfamily ankyrin, member 1 (TRPA1) ion channels, which sense low temperatures. In this study, we examined the effect of rokumigan (RG) on Oxp‐induced cold hypersensitivity. Methods We examined withdrawal response to cold stimulation and analyzed the expression of TRPM8 and TRPA1 mRNA in the 4th–6th lumbar vertebrae dorsal root ganglia of rats treated with RG, orally, at 1 g/kg/day for 12 days and Oxp, i.p., at 4 mg/kg twice per week, using quantitative reverse transcription–polymerase chain reaction. Results The withdrawal response to cold stimulation at 4 and 10°C was significantly improved with RG–Oxp co‐treatment. Overexpression of TRPM8 and TRPA1 mRNA induced by treatment with Oxp was restored to a level similar to that of the vehicle group by co‐treatment with RG. Conclusion The six herbs that constitute RG may potentially alleviate Oxp‐induced cold hypersensitivity. Further research is necessary to analyze the effect of each herbal component of RG on the expression of TRP channels.